Composite
Part:BBa_K3693010:Design
Designed by: Jie Gao Group: iGEM20_BJ101HS (2020-10-22)
Purification of CSN3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We add his-tag at both N-terminal and C-terminal for affinity purification
Source
The his-tag is we designed and added artificially， the gene it self is from Homo sapiens but we did codon optimization.